Veterinary andrology and artificial insemination pdf
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Metrics details. In addition, the suitability of the semen for insemination and its effect on reproduction in females were determined in relation to the time of storage. As the percentage of sperm with DNA and cell membrane damage increased and the mitochondrial membrane potential decreased, there was an increase in AspAT and acrosin activity. Identification of apoptotic changes in sperm by flow cytometry using the annexin assay, the TUNEL assay and evaluation of mitochondrial membrane potential can be recommended for determination of the suitability of raccoon dog semen for artificial insemination. Cytometric methods of semen analysis should also be used to evaluate various extenders of raccoon dog semen and methods of cryopreservation in terms of ensuring sperm viability, fertilization capacity, and suitability for insemination. The growing interest in breeding fur-bearing animals and the demand for articles of fur, including raccoon dog skins, has led to increased work on modern biotechnological methods of reproduction of this species. These measures are aimed at improving the fertility and fecundity of females through the development of new methods of semen collection and storage and the use of artificial insemination [ 1 ].
The popularity of mare insemination as an element affecting the dynamic growth of breeding progress among horses in Europe is subject to various fluctuations. The success of this method of reproduction should be considered first of all in terms of the quality of semen available on the market, the types of semen storage technology, the profitability of its use resulting from the effectiveness of insemination with the selected type of semen, and factors affecting the success of artificial insemination.